This new contract includes the £41m stadium roof contract that Balfour Beatty was awarded last summer. (See previous report here.)
The contract was awarded by park operator E20 Stadium LLP, a joint venture between the London Legacy Development Corporation and Newham Council.
Balfour Beatty will lead the transformation of the stadium into a multi-use venue for sporting events, concerts and shows.
The new venue will host five matches during the Rugby World Cup 2015 and will be the permanent home of West Ham United Football Club from 2016. It will also become the new national stadium for athletics competition in the UK. A new community athletics track will also be provided next to the main stadium.
Sustainable construction methods will include features of the existing facility being recycled and incorporated into the new stadium and the re-use of crushed demolition material, existing balustrades and sanitary ware.
Once reconfigured the stadium's cable net roof, 84m wide at its deepest point, will be the largest cantilevered roof in the world and will cover every stadium seat.
Balfour Beatty will work with WorkPlace, Newham Council's employment service, to ensure the employment of local people where possible. It will have up to 400 people on site at the peak of construction, 7% of whom will be apprentices.
Balfour Beatty chief executive Andrew McNaughton said: "We are delighted to be continuing our activity at the Queen Elizabeth Olympic Park supporting the legacy commitment made as part of the London 2012 Olympic and Paralympic Games.
"During construction, our firm commitment to the use of local labour and the creation of apprenticeships will continue to benefit the local community and the wider industry and, upon completion, the Stadium will provide a first-class sporting and cultural facility for many generations to come. Balfour Beatty is proud to be associated with this project."
Work starts on site this month and is due for completion in the spring of 2016.