The Hampton by Hilton hotel will be built next to London City Airport at the Royal Albert Dock.
GB London Dockside has secured private sector funding, including £20m from Topland, for the scheme.
Brandon Riley, director of hotels at GB Group, said: “This is an exciting development in one of the capital’s prime regeneration locations, and we’re delighted to be working once again with Hilton Worldwide.”
Last year, GB Group secured a mix of public and private sector funding for the £32m Hilton Leeds Arena, currently under construction by GB Building Solutions and due for completion in 2015. GB retains ownership of the Leeds hotel, which is next to Leeds Metropolitan University’s Rose Bowl conference facility and near the Leeds Arena.