The idea is that the funding will help support the enterprise zones in getting businesses to relocate to within their boundaries.
Enterprise zones will compete for a share of the pot. They can submit only one bid each and the deadline for bids is Monday 18 November 2013. Local enterprise partnerships are being asked to coordinate bids in their area.
The money will fund projects such as road building and land clearance that will unlock areas previously idle, said communities secretary Eric Pickles.
Mr Pickles said: “Enterprise zones are at the centre of our plans to back business, help local economies grow stronger and give the UK that competitive edge.
“The zones are showing progress with over 180 businesses, half a billion pounds of private sector investment and nearly 4,000 jobs, and many more in the pipeline.
“We want to help them grow further and faster and this extra £100 million investment will ensure they have the infrastructure in place to successfully attract even more businesses and create many more local jobs.”