The contract runs for an initial five years, with a value of £130m, with possible extensions up to 11 years, taking the value to £450m.
Amey will take responsibility for managing and maintaining Gloucestershire’s highway network, including nearly 10,000 km of roads, as well as providing winter maintenance services and delivering highway improvement schemes.
There will now be a four month mobilisation period to finalise contractual arrangements so that the new contract can start on the 1st April 2014.
Amey already provides refuse and recycling collections, street cleaning and grounds maintenance services to Gloucester City Council.
Chief executive Mel Ewell said: “We are looking forward to further enhancing our services to the residents of Gloucestershire and will draw on our existing footprint in the local area along with our national highways experience to provide high quality, yet cost efficient services for the county.
“We will work closely with the council to help deliver their vision of providing a safe and sustainable transport network.”
The council’s cabinet member for highways, Cllr Vernon Smith, said: “I am confident that in Amey we have appointed a highways contractor that will provide the quality services our county deserves as well as value for money for Gloucestershire’s tax payers."
This year Amey has picked up six major highways contracts with Liverpool City Council, Transport Scotland, the New South Wales state government agency in Australia, the Queensland Government Department in Australia and ASC 6 and ASC 8 for the Highways Agency.